Paper Contents
Abstract
With 14 million new cases and 8.2 million deaths from the disease in 2012, cancer is one of the leading causes of death worldwide. The goal of many initiatives has been to develop novel and efficient cancer treatments. Anticancer medication screening is one of these tactics. Animal models have usually been used to assess standard screening. Nonetheless, certain findings indicate that human clinical outcomes are not always predicted by animal experiments, particularly when it comes to toxicity evaluations. Knight Furthermore, ethical considerations frequently lead to restrictions on the use of animals in research. Partying Given these concerns, using an in vitro model based on cells is a great way to reduce the number and necessity of animal experiments. Cell-based procedures were first established through 2D cell culture. Since 3D in vitro cancer modelsespecially spheroids and organoidsoffer a more realistic depiction of tumor microenvironments than conventional 2D cell cultures, they are being utilized more and more for anticancer drug screening. These three-dimensional models aid in predicting medication resistance and identifying efficacious treatments, which may result in customized care. The most popular in vitro methods used in research labs to screen anticancer medicationsdye exclusion, colorimetric, fluorometric, and luminometric assaysare discussed. This chapter provides an overview of the fundamentals, benefits, and drawbacks of the cell viability tests.
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Copyright © 2025 JAYADURKA. This is an open access article distributed under the Creative Commons Attribution License.
