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In vitro methods to study antibacterial activity

Rohit vijay ugale

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Abstract

Antimicrobial resistance (AMR) has become a significant global public health concern. Identifying resistant microorganisms is essential for selecting effective treatment options and controlling the transmission of resistant strains. This manuscript summarizes existing and upcoming technologies for detecting microbial resistance and highlights the strengths and limitations of each approach.Microbiological culture and susceptibility testing methods, such as Disk Diffusion, Agar well diffusion,Epsilometer (E test),Tube dilution methods, Chromogenic agar media for rapid detection of AMR pathogens have been employed to identify AMR bacteria. We also examine the non conventional automated methods such as (MALDI-TOF) spectroscopy, VITEK-2 System, Microscan walk away method, Fourier transform infrared spectroscopy (FTIR),BD Phoenix automated microbiology system in antimicrobial resistance, we also summarize the future techniques used in microbial resistance (AMR). By understanding the strengths and limitations of these technologies, stakeholdersincluding researchers, healthcare professionals, regulatory bodies, health authorities, financial managers, and patientscan make informed decisions that help prevent the emergence and spread of antibiotic-resistant strains, thereby ultimately enhancing patient safety.

Copyright

Copyright © 2025 Rohit vijay ugale . This is an open access article distributed under the Creative Commons Attribution License.

Paper Details
Paper ID: IJPREMS51100058339
ISSN: 2321-9653
Publisher: ijprems
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