Paper Contents
Abstract
A simple, reproducible and efficient reverse phase high performance liquid chromatographic method was developed for simultaneous determination of Artesunate and Mefloquine in pure form and marketed combined pharmaceutical dosage forms. A column having Symmetry (C18) (150mm x 4.6mm, 5µm) in isocratic mode with mobile phase containing Methanol: Phosphate Buffer (pH-3.8) (28:72% v/v) was used. The flow rate was 1.0 ml/min and effluent was monitored at 252 nm. The retention time (min) and linearity range (ppm) for Artesunate and Mefloquine were (1.791, 3.442min) and (10-30, 10-50), respectively. The method has been validated for linearity, accuracy and precision, robustness and limit of detection and limit of quantitation. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.86µg/ml and 2.58µg/ml for Artesunate and 1.28µg/ml 3.84µg/ml for Mefloquine respectively. The developed method was found to be accurate, precise and selective for simultaneous determination of Artesunate and Mefloquine in bulk and pharmaceutical dosage forms.
Copyright
Copyright © 2026 G. Haritha, Arunabha Mallik, Thallapalli Uddipa, Kadagoni Pravalika. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
