Paper Contents
Abstract
This study evaluates the antioxidant and anti-inflammatory activities of aqueous extracts from Butea monosperma and Calotropis gigantea leaves using multiple in vitro assays. Soxhlet extraction with distilled water was employed to obtain bioactive compounds, which were tested for their ability to scavenge free radicals (DPPH, ABTS, NBT assays), reduce ferric ions (FRAP assay), and inhibit protein denaturation (BSA and egg albumin assays). Both extracts exhibited dose-dependent antioxidant activity, with B. monosperma showing slightly higher efficacy than C. gigantea. At 160 mgmL, B. monosperma achieved 76.5%, 77.9%, 66.1%, and 57.6 mol FeSO Eq.mL in DPPH, ABTS, NBT, and FRAP assays, respectively, compared to C. giganteas 70.4%, 71.4%, 61.4%, and 52.2 mol FeSO Eq.mL. Anti-inflammatory assays showed both extracts inhibited protein denaturation, with B. monosperma and C. gigantea achieving 71.0% and 73.6% inhibition in the BSA assay, and 65.8% and 69.0% in the egg albumin assay at 160 mgmL, respectively, compared to diclofenacs higher inhibition. While less potent than reference standards (ascorbic acid and diclofenac), both extracts demonstrated significant bioactivity, suggesting potential as natural therapeutic agents for oxidative stress and inflammation-related conditions.
Copyright
Copyright © 2025 Apurva Dubey. This is an open access article distributed under the Creative Commons Attribution License.
