Paper Contents
Abstract
Recent developments in pharmaceutical analysis have introduced chromatographic media featuring a 1. 7 m particle size along with a liquid handling system capable of functioning with such columns at significantly higher pressures. This innovation, known as Ultra performance liquid chromatography (UPLC), utilizes sub 2 micron particles and exceptionally high pressure (up to 100 MPa is achievable in a UPLC system) and has shown enhancements in method sensitivity, resolution, and speed in comparison to traditional HPLC. The UPLC system permits a reduction in analysis time by as much as nine times when contrasted with chromatographic systems utilizing 5 m size particle packed analytical columns. When comparing with 3m size particle packed analytical columns, the analysis time is reduced by approximately three times. This review paper highlights the distinctions between HPLC and UPLC, the validation of analytical methods, and the applications, advantages, and disadvantages of both HPLC and UPLC.
Copyright
Copyright © 2025 Aniket Sharad Salunke . This is an open access article distributed under the Creative Commons Attribution License.
