Paper Contents
Abstract
Immobilization of enzymes on solid supports, Silica coated Superparamagnetic Iron OxideNanoparticles (SPIONs) for bio- catalysis has been studied. In this study Candida rugosalipase (CRL) enzyme was immobilized on the surface of SPIONs through chemical bindingprocesses. Characterization of the nanoparticles (NPs) was achieved with the use ofScanning Electron Microscope (SEM) and the magnetic properties were determined usingVibrating Sample Magnetometer (VSM). The NPs were functionalized with 3-aminopropyltriethoxysilane (APTS) thus enabling the enzymes to be chemically bounded on the surface.Free and immobilized enzymes were used in hydrolysis of p-nitrophenyl palmitate (PNPP) toproduce an alcohol, p-nitrophenol (PNP) in order to determine the catalytic efficiency of thelipase enzyme. During the process of hydrolysis, more alcohol was produced at the highestrate with free enzymes in comparison with the immobilized enzyme. However, the efficiencyof enzymes immobilized on functionalized nanoparticles (FNPs) shows recycling ability butdropped after each cycle.
Copyright
Copyright © 2024 Amina Muhammad Ahmad . This is an open access article distributed under the Creative Commons Attribution License.
