Paper Contents
Abstract
The aim of the present study was carried out with the objective of phytochemical screening and to evaluate the hepatoprotective activity of aqueous extract of Desmodium gangeticum using drug induced hepatotoxicity models like Diclofenac induced methods. The rats were divided into five groups with six rats in each. Group I (Control) served as normal and received the vehicle alone (Sterile distilled water, 10 mlkg, p.o.) for 5 days. Group II (Toxin control) animals on the 3rd and 4th day. Group III and IV were treated with DGL at a dose level of 400 mgkg and 600 mgkg body weight p.o. per day respectively for 5 days and on the 3rd and 4th day with hepatotoxic drugs was given 1 h after the treatment of the extract. Group V (Standard) was treated with standard drug silymarin (100 mgkg p.o.) for 5 days and on the 3rd and 4th day hepatotoxic drugs was given 1h after the treatment of the drug. In hepatoprotective studies, the induced Diclofenac toxicity elevated levels of serum marker enzymes ALT, AST, ALP and the level of BUN along with the decrease in total protein and albumin levels. It also increased the relative liver weight and decreased the level of liver total protein and GSH. The activity of catalase and GPx significantly decreased in Diclofenac intoxicated animals. The pre-treatment of methanol extract of Desmodium gangeticum leaves at dose levels of 400 and 600 mgkg had restored the ALT, AST, ALP and BUN levels towards normalization and the effects were comparable with standard drug (Silymarin 100 mgkg). The data obtained from animal experiments are expressed as mean SEM (standard error of mean). For statistical analysis data were subjected to analysis of variance (ANOVA) followed by Student t-test. Values are considered statistically significant at p < 0.01 for ANOVA and P < 0.05 for t-test
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Copyright © 2024 Dr Rangu Nirmala. This is an open access article distributed under the Creative Commons Attribution License.
