Microbial production of L-asparaginase from soil microbe and it's medium optimization for bioremediation of agricultural waste
R. Dharani Dharani
Paper Contents
Abstract
Isolation of Staphylococcus for the Production of L-asparaginase enzyme from bioremediating agricultural waste . From various microorganisms , L-asparaginase enzyme can be produced . These enzymes are helpful in the treatment of leukemia and it is also used in starchy food industries . From soil sample screening of L-asparaginase which is useful for producing bacteria was done . The screening is carried by two methods ie, primary screening and secondary screening. The primary screening is done by qualitative method .here rapid plate assay is used as a qualitative method the secondary screening is done by nesslerization method. The bacterial isolates A6, c11, C19 . Were bacillus species and all were staphylococcus species which is observed pink color zone in primary screening . It was identified based on morphological , cultured and biochemical test. L-asparaginase enzyme that used to make proteins and create a new cells as a medication and in food manufacturing . It is approved for medical use in the United states from 1978 onwards
Copyright
Copyright © 2023 R. Dharani. This is an open access article distributed under the Creative Commons Attribution License.