Paper Contents
Abstract
Pleurotus species are commonly grown mushrooms that are crucial to the global economy. About 25 percent of all grown mushrooms worldwide are produced only from Pleurotus mushrooms. For the mushroom industry, producing high-quality spawn with a good capacity to colonize fruiting substrates with a low risk of contamination is essential. Pleurotus species are easily cultivated because of their 100% biological efficiency and ability to colonize a variety of agro-substrates rapidly. This study aims to optimize mycelium growth and find an approach to immobilizing it. The substrate jars were inoculated with both solid and liquid inoculum and incubated for 10 days in a dark condition at 21 C. The coverage of mycelium in the jars was compared on the 10th day. It was demonstrated that the jars inoculated with liquid broth have covered 95% of the mycelium, while the jars of solid inoculum have only covered 70% of the mycelium. After the result was interpreted, the cells of the liquid broth of P. florida were immobilized using calcium chloride and sodium alginate. Once sodium alginate and fungal broth were added to a calcium chloride solution, spherical, glossy beads with an off-white color were produced. Research on bioremediation, pharmaceuticals, food technology, and environmental sustainability may find uses in these fields. This initiative seeks to leverage the biotechnological potential of Pleurotus florida to provide exciting and sustainable responses to current problems.Keywords: P. florida, mycelium, cell immobilizing substrate, calcium chloride, sodium alginate
Copyright
Copyright © 2024 Rohit Rawat. This is an open access article distributed under the Creative Commons Attribution License.
