Paper Contents
Abstract
The species of the Pterospermum genus have been traditionally used to cure inflammation, blood troubles, leprosy, tumors, hemostatic, ear pain, stomachache, smallpox, leucorrhea, and ulcer. Despite its historical and widespread use, there is a significant gap in our understanding of the plant's toxicological profile. As such, this study aims to comprehensively evaluate the acute, sub-acute, and cutaneous toxicity assessments of the ethyl acetate fraction of P. lanceifolium (EAPL) leaf in experimental rats. The present study involved an array of toxicity assessments, including an acute oral toxicity study by administering a single dose of 2000 mgkg EAPL to rats as per OECD guidelines no- 423. Additionally, a 28-day sub-acute toxicity trial was conducted by administering daily doses of EAPL at 200 and 400 mgkg p.o. as per OECD 407. To assess cutaneous toxicity, rats were exposed to topically applied concentrations of 1% and 5% EAPL over a fourteen-day period, as per OECD 402. Acute oral toxicity testing revealed no lethal effects or behavioral indicators of toxicity at the tested doses, indicating that the LD50 should be greater than 2000 mgkg. The sub-acute study demonstrated a EAPL at 200, 400 mgkg did not impart any significant change in any parameter. Notably, acute dermal toxicity analysis indicated no mortality in rats and no significant change in biochemical parameter when exposed to 1% and 5% EAPL. This study establishes that the oral LD50 (lethal dose, 50%) of the EAPL exceeds 2000 mgkg. The findings further potentiate the safety of EAPL at the tested ethno-medicinal doses and further pave the path for its potential use in traditional and modern therapeutic applications.
Copyright
Copyright © 2025 Sushmita Padhi. This is an open access article distributed under the Creative Commons Attribution License.
