Paper Contents
Abstract
Ganoderma lucidum is a medicinal species that is classified as a white rot fungus that may produce extracellular ligninolytic enzymes. Ganoderma lucidum is a pharmacologically diverse basidiomycete that degrades wood. Due to the mushroom's extreme rarity in the wild, fruiting bodies have been artificially cultivated on wood logs and sawdust in plastic bags or bottles. It has also been studied that G. lucidum mycelia can be biotechnologically cultivated in bioreactors on solid substrates and in liquid media through the submerged cultivation of fungal biomass. The aim of this study was to optimize the mycelial growth of Ganoderma lucidum inoculated on a substrate (wheat) with liquid and solid media. The liquid media inoculated substrate showed enhanced growth and covered 95% of the substrate. While solid culture showed only 50% of the mycelium cover. An interesting finding is the fact that mycelium grown on liquid medium and used as inoculum for substrate provided a 3-fold increase in mycelium coverage in the substrate, and cells from liquid broth were immobilized by the entrapment method to increase its application in various fields. Keywords: G. lucidum, mushroom, mycelium, substrate, fungus
Copyright
Copyright © 2024 Rohit Rawat. This is an open access article distributed under the Creative Commons Attribution License.
